Laboratory Medicine

Biological Centrifugation by Dr John Graham

By Dr John Graham

A major advent to using the centrifuge within the biology laboratory, organic Centrifugation is usually helpful for more matured employees. The e-book describes the history and the foundations at the back of centrifugation, together with sedimentation idea. The ebook additionally considers the differing kinds of centrifuge and different centrifuge to be had, density gradient media and gradient expertise. even if aimed essentially on the amateur, this identify additionally presents details to permit more matured staff to switch and replace present innovations.

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Centrifugation hardware 19 Detailed specifications of a large selection of ultracentrifuge rotors are given in Rickwood (1992a). 4. Uses of centrifuges and rotors It is necessary to make a distinction between the use of centrifuges and centrifuge rotors for (a) halVesting of panicles from culture media, homogenates and cell extracts, in which the selection of the appropriate centrifuge and rotor depends almost solely on the rate of sedimentation of the particles and (b) the subsequent purification or analysis of particles using density gradients which generally require much higher ReFs than those needed for simple pelleting.

LIirns-comaining ftllid, for example, can be pumped inlo the rotor using path (b). m. through path a), itftows up oiler ti,e core of the rotor, allowing the viral particles to sediment out into the gradient From /. Graham (1992) Separations in zonal rotors. In: Preparative Centrifugation - A Practical Approach (ed. D. Ricl

Fixed-angle; NV. near-vertical; V. vertical. 1 Materials Glass centrifuge tubes are still commonly used for low-speed centrifugation, but with the exception of use for work with organic solvents, most operations can be more conveniently carried out in plastic tubes. Ordinaty Pyrex® glass tubes can withstand forces of around 2000 g, while Corex® glass tubes can be centrifuged at up to 12 000 g. Plastic tubes have the advantage of cheapness and can be obtained ready-sterilized (an important consideration for harvesting cultured cells).

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